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 | Acesso ao texto completo restrito à biblioteca da Embrapa Semiárido. Para informações adicionais entre em contato com cpatsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
08/06/2020 |
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Data da última atualização: |
12/06/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CAMPESTRINI, L. H.; RASERA, G. B.; CAMARGO, A. C. de; FRANCHIN, M.; NANI, B. D.; ROSALEN, P. L.; CANNIATTI-BRAZACA, S. G.; BIASOTO, A. C. T.; SHAHIDIC, F.; ALENCAR, S. M. |
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Afiliação: |
Luciano Henrique Campestrini; Gabriela Boscariol Rasera; Adriano Costa de Camargo; Marcelo Franchin; Bruno Dias Nani; Pedro Luiz Rosalen; Solange Guidolin Canniatti-Brazaca; ALINE TELLES BIASOTO MARQUES, CPATSA; Fereidoon Shahidic; Severino Matias Alencar. |
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Título: |
Alkaline conditions better extract anti-inflammatory polysaccharides from winemaking by-products. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Food Research International, v. 131, may 2020. |
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DOI: |
https://doi.org/10.1016/j.foodres.2019.108532 |
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Idioma: |
Inglês |
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Conteúdo: |
Winemaking generates large amounts of by-products, a well recognized source of phenolic compounds. However, less attention has been paid to the polysaccharide-rich fraction (PRF) and effects of fractionation techniques on its potential bioactivity. Therefore, PRFs from Syrah and Tempranillo winemaking by-products were extracted under aqueous (neutral pH conditions), acidic and alkaline conditions. PRFs were screened for their monosaccharide composition, uronic acid content, homogeneity and molecular weight. Anti-inflammatory activity of PRFs were evaluated on stimulated RAW 264.7 macrophages. PRF obtained in water and/or under acidic conditions showed heterogeneous profiles. As like as in the others, a heterogeneous and complex profile was detected in extracts procured under alkaline conditions. A high content of uronic acid was found in aqueous extracts, thus indicating the presence of pectin. Pectin and hemicellulose were present in PRFs procured under acidic conditions. Alkaline conditions rendered extracts containing a complex mixture of monosaccharides, mainly xylose. This latter PRF was the only one exhibiting anti-inflammatory potential (at 100 ?g/mL) by reducing the release of TNF-? and activation of NF-?B in LPS-activated RAW 264.7 macrophages, with no effect on cell viability. Regardless of the grape variety, PRFs obtained under alkaline conditions were the best option to obtain bioactive polysaccharides with potential application as a source of anti-inflammatory compounds. A complex mixture of polymers may be responsible for the anti-inflammatory effects. Finally, according to results procured by NMR, it is possible to suggest that bioactive fractions are composed of a chain of ?-L-Araf-(1 ? 3) linked, ?-D-Xylp- (1 ? 4), ?-D-Glcp-(1 ? 4) linked, ?-D-GalpA-(1 ? 4), ?-D-Gal-(1 ? 2) forming possible RG I and RG II and xylan chains. MenosWinemaking generates large amounts of by-products, a well recognized source of phenolic compounds. However, less attention has been paid to the polysaccharide-rich fraction (PRF) and effects of fractionation techniques on its potential bioactivity. Therefore, PRFs from Syrah and Tempranillo winemaking by-products were extracted under aqueous (neutral pH conditions), acidic and alkaline conditions. PRFs were screened for their monosaccharide composition, uronic acid content, homogeneity and molecular weight. Anti-inflammatory activity of PRFs were evaluated on stimulated RAW 264.7 macrophages. PRF obtained in water and/or under acidic conditions showed heterogeneous profiles. As like as in the others, a heterogeneous and complex profile was detected in extracts procured under alkaline conditions. A high content of uronic acid was found in aqueous extracts, thus indicating the presence of pectin. Pectin and hemicellulose were present in PRFs procured under acidic conditions. Alkaline conditions rendered extracts containing a complex mixture of monosaccharides, mainly xylose. This latter PRF was the only one exhibiting anti-inflammatory potential (at 100 ?g/mL) by reducing the release of TNF-? and activation of NF-?B in LPS-activated RAW 264.7 macrophages, with no effect on cell viability. Regardless of the grape variety, PRFs obtained under alkaline conditions were the best option to obtain bioactive polysaccharides with potential application as a source of anti-inflammatory c... Mostrar Tudo |
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Palavras-Chave: |
Atividade anti-inflamatória; Extração sequencial; Polissacarídeos; Vinificação. |
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Thesagro: |
Pectina; Subproduto; Uva. |
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Thesaurus Nal: |
Grapes; Polysaccharides. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02892naa a2200349 a 4500
001 2123132
005 2020-06-12
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.foodres.2019.108532$2DOI
100 1 $aCAMPESTRINI, L. H.
245 $aAlkaline conditions better extract anti-inflammatory polysaccharides from winemaking by-products.$h[electronic resource]
260 $c2020
520 $aWinemaking generates large amounts of by-products, a well recognized source of phenolic compounds. However, less attention has been paid to the polysaccharide-rich fraction (PRF) and effects of fractionation techniques on its potential bioactivity. Therefore, PRFs from Syrah and Tempranillo winemaking by-products were extracted under aqueous (neutral pH conditions), acidic and alkaline conditions. PRFs were screened for their monosaccharide composition, uronic acid content, homogeneity and molecular weight. Anti-inflammatory activity of PRFs were evaluated on stimulated RAW 264.7 macrophages. PRF obtained in water and/or under acidic conditions showed heterogeneous profiles. As like as in the others, a heterogeneous and complex profile was detected in extracts procured under alkaline conditions. A high content of uronic acid was found in aqueous extracts, thus indicating the presence of pectin. Pectin and hemicellulose were present in PRFs procured under acidic conditions. Alkaline conditions rendered extracts containing a complex mixture of monosaccharides, mainly xylose. This latter PRF was the only one exhibiting anti-inflammatory potential (at 100 ?g/mL) by reducing the release of TNF-? and activation of NF-?B in LPS-activated RAW 264.7 macrophages, with no effect on cell viability. Regardless of the grape variety, PRFs obtained under alkaline conditions were the best option to obtain bioactive polysaccharides with potential application as a source of anti-inflammatory compounds. A complex mixture of polymers may be responsible for the anti-inflammatory effects. Finally, according to results procured by NMR, it is possible to suggest that bioactive fractions are composed of a chain of ?-L-Araf-(1 ? 3) linked, ?-D-Xylp- (1 ? 4), ?-D-Glcp-(1 ? 4) linked, ?-D-GalpA-(1 ? 4), ?-D-Gal-(1 ? 2) forming possible RG I and RG II and xylan chains.
650 $aGrapes
650 $aPolysaccharides
650 $aPectina
650 $aSubproduto
650 $aUva
653 $aAtividade anti-inflamatória
653 $aExtração sequencial
653 $aPolissacarídeos
653 $aVinificação
700 1 $aRASERA, G. B.
700 1 $aCAMARGO, A. C. de
700 1 $aFRANCHIN, M.
700 1 $aNANI, B. D.
700 1 $aROSALEN, P. L.
700 1 $aCANNIATTI-BRAZACA, S. G.
700 1 $aBIASOTO, A. C. T.
700 1 $aSHAHIDIC, F.
700 1 $aALENCAR, S. M.
773 $tFood Research International$gv. 131, may 2020.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
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Registro Completo
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
14/10/2011 |
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Data da última atualização: |
17/10/2011 |
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Autoria: |
PELISSARI, A. L.; LANSSANOVA, L. R.; DRESCHER, R. |
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Afiliação: |
ALLAN LIBANIO PELISSARI, UFMT; LUCIANO RODRIGO LANSSANOVA, UFMT; RONALDO DRESCHER, UFMT. |
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Título: |
Modelos volumétricos para Pinus tropicais, em povoamento homogêneo, no Estado de Rondônia. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Pesquisa Florestal Brasileira, Colombo, v. 31, n. 67, p. 173-181, jul./set. 2011. |
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DOI: |
10.4336/2011.pfb.31.67.173 |
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Idioma: |
Português |
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Conteúdo: |
Este trabalho teve como objetivo ajustar modelos volumétricos para Pinus tropicais, em povoamento homogêneo, no Município de Vilhena, RO. Para a coleta de dados, foram derrubadas 20 árvores de Pinus caribaea var. hondurensis e 10 de Pinus tecunumanii, sendo coletados discos em posições fixas de 0,20 m; 0,70 m; 1,30 m e em distâncias de um metro ao longo do fuste, para a posterior contagem e mensuração dos anéis de crescimento nas idades de 4 a 12 anos. Foram ajustados oito modelos volumétricos e o critério de seleção considerou os resultados do erro padrão da estimativa, coeficiente de determinação ajustado, teste F, significância dos coeficientes de regressão, desvio médio, desvio-padrão das diferenças, soma de quadrados do resíduo relativo, resíduo percentual e a análise ráfica dos resíduos. Os modelos de Näslund modificado e Spurr apresentaram, respectivamente, o melhor ajuste para a estimativa do volume nos povoamentos de Pinus caribaea var. hondurensis, submetido ao primeiro desbaste, e Pinus tecunumanii, com idades entre 4 e 12 anos, em Vilhena, RO. |
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Palavras-Chave: |
Análise de tronco; Equação volumétrica. |
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Thesagro: |
Volume. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/43357/1/Modelos-volumetricos-para-Pinus-tropicais-em-povoamento-homogeneo-no-Estado-de-Rondonia.pdf
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Marc: |
LEADER 01731naa a2200193 a 4500
001 1903116
005 2011-10-17
008 2011 bl uuuu u00u1 u #d
024 7 $a10.4336/2011.pfb.31.67.173$2DOI
100 1 $aPELISSARI, A. L.
245 $aModelos volumétricos para Pinus tropicais, em povoamento homogêneo, no Estado de Rondônia.$h[electronic resource]
260 $c2011
520 $aEste trabalho teve como objetivo ajustar modelos volumétricos para Pinus tropicais, em povoamento homogêneo, no Município de Vilhena, RO. Para a coleta de dados, foram derrubadas 20 árvores de Pinus caribaea var. hondurensis e 10 de Pinus tecunumanii, sendo coletados discos em posições fixas de 0,20 m; 0,70 m; 1,30 m e em distâncias de um metro ao longo do fuste, para a posterior contagem e mensuração dos anéis de crescimento nas idades de 4 a 12 anos. Foram ajustados oito modelos volumétricos e o critério de seleção considerou os resultados do erro padrão da estimativa, coeficiente de determinação ajustado, teste F, significância dos coeficientes de regressão, desvio médio, desvio-padrão das diferenças, soma de quadrados do resíduo relativo, resíduo percentual e a análise ráfica dos resíduos. Os modelos de Näslund modificado e Spurr apresentaram, respectivamente, o melhor ajuste para a estimativa do volume nos povoamentos de Pinus caribaea var. hondurensis, submetido ao primeiro desbaste, e Pinus tecunumanii, com idades entre 4 e 12 anos, em Vilhena, RO.
650 $aVolume
653 $aAnálise de tronco
653 $aEquação volumétrica
700 1 $aLANSSANOVA, L. R.
700 1 $aDRESCHER, R.
773 $tPesquisa Florestal Brasileira, Colombo$gv. 31, n. 67, p. 173-181, jul./set. 2011.
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Registro original: |
Embrapa Florestas (CNPF) |
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